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用人血清白蛋白诱发ｗｉｓｔａｒ大鼠肝脏纤维化模型。成功率可达８９．２３％。不同温度对成功率有显著影响（Ｐ＜０．０１）。同时证明，，不同日龄大鼠对成功率亦有影响。前列腺素ＥＩ（ＰＧＥＩ）的使用是本模型建立的技术关键，形态学观察，纤维化后细胞损伤轻微，汇管区纤维细胞增生明显。有假小叶形成。电镜观察见枯否氏细胞活跃，Ｄｉｓｓｃ腔间隙增宽，多量胶原纤维形成，肝血窦周围贮脂细胞呈增生状态，并见贮脂细胞内胶原纤维形成的现象。细胞组织化学检查，粗面内质网葡萄糖－６－磷酸酶（Ｇ－６６Ｐ酶）颗粒阴性，致密体，自噬泡酸性磷酸酶（ＡＣＰ）反应阳性；胞膜及胆小管５－核苷酸酶（５－Ｎ酶）反应阳性。
Induction of hepatic fibrosis in wistar rats with human serum albumin. The success rate of up to 89.23%. Different temperatures have a significant impact on the success rate (P <0.01). At the same time prove that different days of age rats also have an impact on the success rate. The use of prostaglandin EI (PGEI) was the key technique in this model. Morphology was observed. The cell damage was slight after fibrosis and the proliferation of fibroblasts in portal area was obvious. False lobule formation. Electron microscopy showed Kupffer cell activity, Dissc cavity gap widened, the formation of large amounts of collagen fibers, fat storage cells around the sinusoid hyperplasia, and see the storage of collagen fibers in the formation of the phenomenon. Cell histochemical examination revealed that the rough endoplasmic reticulum glucose-6-phosphatase (G-66P) was negative, dense and autopholytic acid phosphatase (ACP) positive. The membrane and tubule 5- Acid enzyme (5-N enzyme) reaction was positive.