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以尾细桉无菌生根苗叶片为外植体,通过调整培养基中TDZ、NAA和磷酸二氢钾浓度和外植体状态和部位进行诱导植株再生研究,以期建立起尾细桉叶片诱导的再生体系。结果表明:TDZ、NAA和磷酸二氢钾浓度对叶片诱导植株再生呈显著性影响,同时外植体状态和部位对叶片诱导植株再生也起到明显的作用。通过试验,最终得到在0.025 mg/L TDZ+0.05 mg/L NAA加入170 mg/L的磷酸二氢钾的培养基中,同时以在生根培养基上生长25 d生根苗顶端起向下1-4片叶为外植体获得了最高72.7%的植株再生效率,诱导芽为丛芽,芽再生部位为叶柄和叶脉中伤口部位,外植体平均芽数达到14.14个,初步建立了尾细桉叶片诱导的遗传转化体系,本研究为下一步尾细桉高效稳定遗传转化体系研究提供研究基础。
Taking the leaves of Asepsculus erecta as explants, the regeneration of plantlets was induced by adjusting the concentration of TDZ, NAA and potassium dihydrogen phosphate (KH2PO4) in the medium and the state and location of the explants. Regeneration system. The results showed that the concentrations of TDZ, NAA and potassium dihydrogen phosphate had significant effects on plant regeneration induced by leaves, and the explant status and site also played an important role in plant regeneration induced by leaves. The results showed that when the rooting medium of 0.025 mg / L TDZ + 0.05 mg / L NAA was added to 170 mg / L potassium dihydrogen phosphate, 4 leaves as the explants to obtain the highest plant regeneration efficiency of 72.7%, inducing shoots buds, buds regenerated parts of the petiole and veins in the wound site, the average number of explants buds reached 14.14, the initial establishment of Eucalyptus urophylla Leaf-induced genetic transformation system, this study provides the research foundation for the next step Eucalyptus urophylla efficient and stable genetic transformation system.