论文部分内容阅读
目的：为应用ｔＰＡ单克隆抗体建立ＥＬＩＳＡ法检测ｔＰＡ含量以及研究ｔＰＡ功能和结构的关系。方法：运用杂交瘤技术成功地研制５株ｔＰＡ单克隆抗体，并进行较系统的免疫特性的鉴定。结果：５株单抗特异性高，与ｕＰＡ、ＰＬＧ、Ｆｇ、Ｆｂ、ＢＳＡ均无交叉反应；亲合力强１Ｈ４＞３Ｃ１０＞５Ｈ１０＞４Ｅ６＞４Ｃ６；腹水效价５×１０－６～１×１０－７；免疫球蛋白亚类为ＩｇＧ１和ＩｇＧ２ａ；５株单抗中，３Ｃ１０和１Ｈ４可明显抑制ｔＰＡ活性，而５Ｈ１０、４Ｅ６、４Ｃ６则对ｔＰＡ活性无明显影响。结论：为进一步应用这些单抗作为研究手段提供了基础。
OBJECTIVE: To establish an ELISA assay for the content of t-PA and to study the relationship between t-PA function and structure for the application of t-PA monoclonal antibody. Methods: Five monoclonal antibodies against tPA were successfully developed by hybridoma technique and identified more systematically. Results: The 5 McAbs were highly specific and had no cross reaction with uPA, PLG, Fg, Fb and BSA. The avidity was 1H4> 3C10> 5H10> 4E6> 4C6. The ascites titer was 5 × 10-6 ~ 1 × 10-7; immunoglobulin subclass IgG1 and IgG2a; 5 monoclonal antibody, 3C10 and 1H4 can significantly inhibit t-PA activity, while 5H10, 4E6, 4C6 T-PA activity had no significant effect. Conclusion: This study provides the basis for further application of these mAbs as research tools.