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目的:探讨CD133+细胞的生物学特性,分析CD133作为胶质瘤干细胞标记的可行性。方法:利用免疫荧光染色检测U251细胞中CD133、巢蛋白(nestin)、神经元特异性烯醇化酶(NSE)、神经胶质细胞纤维酸性蛋白(GFAP)等蛋白表达,分析CD133+细胞与CD133-细胞中巢蛋白、NSE、GFAP阳性细胞存在的差异。结果:U251中CD133+细胞比例为0.060±0.003,在CD133+细胞中,巢蛋白阳性细胞,NSE和GFAP阴性细胞比CD133-中明显增多;在CD133-细胞中,也存在巢蛋白阳性细胞,数目较CD133+中明显减少,相反巢蛋白阴性细胞,NSE和GFAP阳性细胞明显增多。结论:胶质瘤U251细胞存在CD133+细胞,在CD133阳性和阴性细胞中,均存在干细胞样细胞,但是在CD133+细胞中此类细胞更多。
Objective: To investigate the biological characteristics of CD133 + cells and to analyze the feasibility of using CD133 as a marker of glioma stem cells. Methods: The expression of CD133, nestin, neuron specific enolase (NSE) and glial fibrillary acidic protein (GFAP) in U251 cells were detected by immunofluorescence staining. The expressions of CD133 + CD133- Differences in Nestin, NSE, GFAP-positive cells. Results: The percentage of CD133 + cells in U251 was 0.060 ± 0.003. The number of nestin positive cells, NSE and GFAP negative cells in CD133 + cells was significantly higher than that in CD133-. In CD133- cells, there were also nestin positive cells, In contrast, significantly decreased nestin negative cells, NSE and GFAP positive cells. CONCLUSIONS: CD133 + cells exist in glioma U251 cells. Stem cell-like cells exist in both CD133 positive and negative cells, but more in CD133 + cells.