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建立了胶束动电毛细管色谱法分离和测定晶福眼药水中烟酰胺、腺苷和硫柳汞含量的测定方法．以十二烷基磺酸钠（７５ｍｍｏｌ／Ｌ）为胶束相，运行液为２０ｍｍｏｌ／Ｌ硼酸缓冲液（ｐＨ＝９．２），恒定电压２０ｋＶ，检测波长２６０ｎｍ；６ｍｉｎ内三组分达到基线分离．选用Ｌ－色氨酸作内标物进行测定，各组分线性范围：烟酰胺５～１０００ｍｇ／Ｌ（ｒ＝０．９９９６），腺苷５～３００ｍｇ／Ｌ（ｒ＝０．９９９９），硫柳汞２～２５０ｍｇ／Ｌ（ｒ＝１．００００）；平均回收率±ＲＳＤ：烟酰胺（９８．４±０．８）％，腺苷（１０１．０±１．１）％，硫柳汞（１００．４±０．３）％，ｎ＝３．此法简便快速，重现性好．
Micellar electrokinetic capillary chromatography was established for the determination of nicotinamide, adenosine and thimerosal in Jingfu eye drops. With sodium dodecyl sulfate (75mmol / L) as micellar phase, the running solution was 20mmol / L borate buffer (pH = 9.2), constant voltage was 20kV and the detection wavelength was 260nm. Separated. L-tryptophan was selected as internal standard to determine the linear range of each component: nicotinamide 5 ~ 1000mg / L (r = 0.9996), adenosine 5 ~ 300mg / L The average recovery was ± RSD: niacinamide (98.4 ± 0.8)%, adenosine (101.0 ± 1.1)%, thimerosal (100.4 ± 0.3)%, n = 3. This method is simple, fast and reproducible.