论文部分内容阅读
目的研究芎芍胶囊含药血清对人脐静脉内皮细胞(HUVEC)的增殖作用。方法采用血清药理学和体外培养HUVEC的方法,通过倒置显微镜观察、MTT法和流式细胞术(FCM)检测芎芍胶囊高、中、低3个浓度含药血清对HUVEC增殖的影响。结果MTT法检测结果显示高、中、低剂量的芎芍胶囊含药血清均有促进HUVEC增殖的作用,增殖率分别为54.14%、54.98%、33.77%;FCM法检测发现高、中、低剂量芎芍胶囊含药血清均可增加S期细胞比例,分别为52.14%、42.65%、37.08%;显微镜观察结果也显示各含药血清具有促进HUVEC增殖的作用。结论芎芍胶囊能促进HUVEC的增殖,其促进细胞增殖的作用与浓度有一定的关系;提示芎芍胶囊的促血管新生作用可能是通过促进血管内皮细胞的增殖。
Objective To study the proliferation of human umbilical vein endothelial cells (HUVECs) treated with drug-containing serum of Xiongshao capsule. Methods Serum pharmacology and in vitro cultured HUVEC were used to detect the effect of Xiongshao capsule on the proliferation of HUVEC by inverted microscopy, MTT assay and flow cytometry (FCM). Results The results of MTT assay showed that high, medium and low doses of the drug-containing serum of Xiongshao capsule could promote the proliferation of HUVEC with the proliferation rates of 54.14%, 54.98% and 33.77%, respectively. The results of FCM showed that high, medium and low dose The drug-containing serum of Xiongshao capsule could increase the proportion of S-phase cells, which were 52.14%, 42.65% and 37.08% respectively. The results of microscopic examination also showed that each drug-containing serum could promote the proliferation of HUVEC. Conclusion Xiongshao capsule can promote the proliferation of HUVEC and promote the proliferation of cells with a certain concentration relationship; suggesting that the role of Xiongshao capsule angiogenesis may be through the promotion of vascular endothelial cell proliferation.