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目的 :进一步探讨肾脏疾病患者血清中抗中性粒细胞细胞质抗体 (ANCA)的荧光模式及其意义。方法 :应用间接免疫荧光(IIF)和ELISA方法检测了 2 5 1例肾脏疾病患者血清中ANCA及其靶抗原。结果 :在 2 5 1例肾脏疾病患者中 ,ANCA阳性 5 1例占 2 0 .3% ,其中 10例为cANCA阳性 (10 / 2 5 1) ;30例为pANCA阳性 (30 / 2 5 1) ;11例为aANCA阳性 (11/ 2 5 1)。结论 :同时用IIF法和ELISA法检测ANCA ,可以提高ANCA检出的阳性率 ;不同ANCA荧光模式与疾病的种类病情及预后密切相关 ;提高对不典型ANCA和 pANCA伴ANA荧光模式的鉴别 ,有助于临床对血管炎和其它自身免疫性疾病的诊断
Objective: To further explore the fluorescence pattern of serum anti-neutrophil cytoplasmic antibody (ANCA) in patients with kidney disease and its significance. Methods: Serum ANCA and its target antigen in 251 patients with renal disease were detected by indirect immunofluorescence (IIF) and ELISA. RESULTS: Among 251 patients with renal disease, ANCA positive was 20.3%, of which 10 were positive for cANCA (10/251) and 30 were pANCA positive (30/251) ; 11 cases were aANCA positive (11/225). CONCLUSION: Both ANF and ELISA assays can improve the positive rate of ANCA. Different patterns of ANCA fluorescence are closely related to the disease status and prognosis. To improve the identification of atypical ANCA and pANCA with ANA fluorescence patterns, To help clinical diagnosis of vasculitis and other autoimmune diseases