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目的调查虎纹蛙自然感染裂头蚴的情况,取裂头蚴感染实验鼠和家犬,为实验教学提供依据。方法解剖虎纹蛙收集裂头蚴,将裂头蚴感染昆明鼠和BALB/c小鼠(6条/只)和SD大鼠(10条/只),对照组灌注生理盐水;60 d后解剖实验动物,收集小鼠体内裂头蚴,并取裂头蚴寄生部位组织包埋切片。将虎纹蛙和鼠体内收集的裂头蚴分别感染家犬6条/只,在感染46 d后取粪便检查,感染60 d后解剖家犬。结果野生虎纹蛙自然感染率为40.63%(403/992);解剖实验组昆明鼠、BALB/c小鼠和SD大鼠肉眼可见皮下肌肉组织有弥漫性瘀血斑点和包块形成,镜下观察见裂头蚴周围有囊壁包绕,纤维组织增生;家犬粪检查到曼氏迭宫绦虫卵,解剖家犬收集到曼氏迭宫绦虫成虫。结论广州市花都区野生虎纹蛙裂头蚴的感染率较高;昆明鼠、BALB/c小鼠和SD大鼠均可作为曼氏迭宫绦虫的转续宿主;家犬是该虫适宜的终末宿主。
Objective To investigate the natural infection of fissure larvae of the tiger frog, and to obtain the evidence that the fission oocysts infect the experimental rats and domestic dogs. Methods Tissue larvae were collected to dissect the sporozoites. Kunming mice and BALB / c mice (6 rats / rat) and SD rats (10 mice / rats) were infected with sporozoite. The control rats were infused with saline. After 60 days, the animals were harvested and collected Mice in vivo spider larvae and sporozoite tissue taken parasitic tissue sections. Six rabbits were infected with sparganum collected from the tiger frogs and mice, respectively. The stools were examined after 46 days and the dogs were dissected 60 days after infection. Results The natural infection rate of wild tiger frogs was 40.63% (403/992). The anatomical groups showed diffuse blood stasis spots and mass formation in the Kunming mice, BALB / c mice and SD rats. Survival observed around the spider labyrinthine wall surrounded by fibrous tissue proliferation; house dog manure to check the Mann’s Tendon eggs, anatomical dogs were collected Manning’s Tendon adult. Conclusions The infection rate of spider larvae of wild tiger frogs in Huadu District of Guangzhou City is high. Kunming mice, BALB / c mice and SD rats can be used as the re-growth hosts of T. mansoni. End host.