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目的建立不同暴露时间点大鼠间歇重度低氧模型,探讨间歇重度低氧对大鼠学习记忆功能的影响。方法成年雄性W istar大鼠48只分为对照组和间歇性低氧组;采用低氧舱模拟5%间歇低氧环境。在间歇低氧2、4、6、8周采用Morris水迷宫检测学习记忆功能,苏木精-伊红(HE)染色观察海马区神经细胞形态变化。结果与对照组比较,间歇性低氧组中神经细胞形态结构损伤明显,存活神经元密度(13.18±2.18)随低氧时间延长降低(P<0.05);低氧2、4、6、8周大鼠逃避潜伏期时间分别为(49.17±8.87)、(58.47±6.98)、(65.15±7.44)和(68.42±7.91)s,随低氧时间延长动物逃避潜伏期时间延长(P<0.5)。结论间歇性低氧可造成神经细胞损伤、学习记忆功能障碍,且随间歇性低氧时间延长而加重。
Objective To establish a rat model of intermittent severe hypoxia at different exposure time points to investigate the effect of intermittent severe hypoxia on learning and memory in rats. Methods Forty-eight adult male Wistar rats were divided into control group and intermittent hypoxia group. The hypoxic chamber was used to simulate 5% intermittent hypoxic environment. Morris water maze was used to detect the learning and memory abilities in intermittent hypoxia for 2, 4, 6 and 8 weeks. The morphological changes of hippocampal neurons were observed by hematoxylin-eosin staining. Results Compared with the control group, the morphological and structural damage of neurons in intermittent hypoxia group was obvious. The density of surviving neurons (13.18 ± 2.18) decreased with the prolongation of hypoxia (P <0.05) The escape latency of rats was (49.17 ± 8.87), (58.47 ± 6.98), (65.15 ± 7.44) and (68.42 ± 7.91) s, respectively. The duration of escape latency was prolonged with hypoxia (P <0.5). Conclusion Intermittent hypoxia can cause nerve cell injury, learning and memory dysfunction, and aggravate with intermittent hypoxia.