论文部分内容阅读
目的:采用蛋白质组学技术,探究中等强度运动后大鼠左室肌蛋白质组的差异表达。方法:将SD大鼠分为运动组和对照组,训练完成后麻醉处死,计量大鼠心重,提取左室肌全蛋白样品,采用2-DE进行分离,选择运动后差异表达量≥10倍的备选目标蛋白点,进行质谱鉴定。结果:取5个目标蛋白点,经质谱鉴定4个。未知蛋白质训练后表达量下调≥12.5倍。结论:1)中等强度运动后,大鼠心脏发生形态学变化使心肌增厚,心收缩力增强;2)训练组大鼠在长期运动训练中,左心室心肌蛋白质组变化显著;3)鉴定出运动医学中还未涉及的4种蛋白质,分别是三磷酸腺苷合酶偶合因子6(CF6)、葡萄糖调节蛋白78(GRP78)、烯酰辅酶A水合酶(ECH)和ATP合酶α亚基(ATP synthase subunit alpha),其表达发生的顺应性改变说明,中等强度运动可提高心肌的能量代谢水平。
OBJECTIVE: To investigate the differential expression of left ventricular muscle proteome in rats after moderate-intensity exercise using proteomic techniques. Methods: Sprague - Dawley rats were divided into exercise group and control group. After training, anesthesia was sacrificed and heart weight was measured. Total protein of left ventricular muscle samples were extracted and separated by 2 - DE. Alternative target protein spots were identified by mass spectrometry. Results: Five target protein spots were identified and identified by mass spectrometry. Unknown protein expression decreased after training ≥ 12.5 times. Conclusions: 1) The morphological changes of the heart of rats after myocardial exercise resulted in thickened myocardium and increased cardiac contractility; 2) In long-term exercise training, the proteome of left ventricular myocardium changed significantly; 3) The four proteins not involved in sports medicine are ATP6, GRP78, ECH and ATP synthase (ATP synthase) subunit alpha), its expression changes in the compliance instructions, moderate-intensity exercise can improve myocardial energy metabolism.