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目的 利用人肝癌细胞株HepG2观察茶多酚和茶色素对端粒酶活性的影响。方法采用TRAP PCR ELISA方法对HepG2细胞端粒酶活性进行定性和定量检测。结果 TRAP PCR定性分析表明空白对照组、茶多酚组和茶色素组均端粒酶阳性 ;ELISA定量结果显示 ,5 0mg/L和 10 0mg/L茶多酚组端粒酶活性 (A450 690 值 )分别为 1 5 6和 1 4 6 ;5 0mg/L和 10 0mg/L茶色素处理组为 1 5 5和1 4 9,与空白对照组 (A450 690 值为 2 11)相比 ,茶多酚和茶色素处理后HepG2细胞端粒酶活性有统计学意义。结论 茶多酚和茶色素显著抑制HepG2细胞端粒酶活性 ,端粒酶活性可能是癌症化学预防研究的一个有用的生物标志物
Objective To observe the effect of tea polyphenols and tea pigments on telomerase activity in HepG2 cells. Methods TRAP PCR was used to detect the telomerase activity in HepG2 cells. Results The results of TRAP PCR showed that telomerase activity was positive in blank control group, tea polyphenol group and tea pigment group. The results of ELISA showed that the telomerase activity (A450 690 value) of tea polyphenols of 50 mg / L and 100 mg / L ) Were 165 and 146 respectively, while those in the 50 mg / L and 100 mg / L tea pigment treatments were 155 and 149, respectively. Compared with the blank control group (A450 690 value 2 11) The telomerase activity of HepG2 cells treated with phenol and tea pigment was statistically significant. Conclusion Tea polyphenols and tea pigments can significantly inhibit telomerase activity in HepG2 cells, and telomerase activity may be a useful biomarker for chemoprevention of cancer