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Objective:To study thei nhibitory effect of purified trichosanthin component on the proliferation of malignant melanoma.Methods:The effect of purified trichosanthin componenton the DNA synthesis,cell cycle and cell apoptos is of murinemelanomacellsweredetected by flow cytometry when culture dinvitro.Results:The significantG0/G1phasearrest was revealed by the inc rease of cellsinG0/G1 phase and decrease of cell sinS phase.The obviousapoptosis of melanoma cells was in duced bypuri fied trichos anthin component.G0/G1phase arrest was high lycorrelated withapop to sis(r=0.8705).Conclusion:The purified trichos anthin component can markedly inhibit melanoma cells by the suppression of DNA syn the sisin S phaseand cell mitosisas well as induction of cell apoptosis.
Objective: To study the inhibition effect of purified trichosanthin component on the proliferation of malignant melanoma. Methods: The effect of purified trichosanthin componenton the DNA synthesis, cell cycle and cell apoptos is of murine melanomacellsweredetected by flow cytometry when culture dinvitro.Results: The significantG0 / G1phasearrest was revealed by the inc rease of cellsinG0 / G1 phase and decrease of cell sinSphase.The obviousapoptosis of melanoma cells was in duced bypuri fied trichos anthin component. G0 / G1phase arrest was high lycorrelated withapop to sis (r = 0.8705) .Conclusion : The purified trichos anthin component can markedly inhibit melanoma cells by the suppression of DNA syn the sisin S phase and cell mitosis as well as induction of cell apoptosis.