目的研究基于预成酶谱分析和涂片染色多功能检查的阴道菌群多维度评价方法在妇科感染诊治中的临床应用价值。方法选取到该院做妇科白带检查的21 175例患者作为研究对象,同一患者均同时采集白带标本做生化检查和涂片染色检查,生化检查采用新型预成酶谱分析技术检测,涂片染色多项检查采用妇科白带涂片多功能染色液染色镜检。结果预成酶谱生化五项阳性检出率:过氧化氢(H2O2)63.83%、唾液酸苷酶(SNa)7.73%、白细胞酯酶(LE)46.16%、β-葡萄糖醛酸苷酶(GUS)3.19%、凝固酶(GADP)46.16%;涂片染色多项检查的各项检出率:滴虫0.63%、念珠菌11.73%、淋球菌0.29%、纤毛菌2.63%、球菌1.82%、条件致病小杆菌17.00%、加特纳球杆菌24.89%、线索细胞17.18%、癌细胞0.23%、阴道清洁度Ⅲ~Ⅳ20.72%。结论联合妇科白带预成酶谱分析与涂片染色多功能检查可在短时间内检出女性常见下生殖道感染、宫颈炎、宫颈癌等异常情况,为全面诊断和治疗妇科疾病提供可靠依据,在大型妇科普查和癌症筛查方面具有重要的临床价值,尤其值得在基层医院推广应用。 Objective To study the clinical value of multidimensional evaluation of vaginal flora based on the pre-enzyme zymogram analysis and smear multi-function test in the diagnosis and treatment of gynecological infection. Methods A total of 21 175 patients with leuco-gynecological examination in our hospital were enrolled in the study. The same patient was also collected for biochemical examination and smear staining of leucorrhea specimens. The biochemical tests were performed by a new pre-enzymatic profiling technique. Check with gynecological vaginal smear multi-purpose staining staining. Results The positive rate of biochemical analysis of preformed biochemical spectrum was 63.83% for hydrogen peroxide (H2O2), 7.73% for SNa, 46.16% for leukocyte esterase (LE), GUS ) 3.19%, coagulase (GADP) 46.16%; smear staining multiple detection of the various detection rates: Trichomonas 0.63%, Candida 11.73%, Neisseria gonorrhoeae 0.29%, 2.63% Cilia, bacteria 1.82%, conditions 17.00% of pathogenic small bacilli, 24.89% of Gardnerella cocci, 17.18% of clue cells, 0.23% of cancer cells, and the vaginal cleanliness was from Ⅲ to Ⅳ 20.72%. Conclusions The combined gynecological leucorrhea pre-enzymatic profiling and smear multi-function examination can detect abnormal female reproductive tract infections such as genital tract infection, cervicitis and cervical cancer in a short time and provide a reliable basis for comprehensive diagnosis and treatment of gynecological diseases. In the large gynecological census and cancer screening has important clinical value, especially worthy of promotion and application in primary hospitals.