【摘 要】
:
通过对30只健康鸡单剂量口服和静注二氟沙星,建立了测定不同时间血浆二氟沙星浓度的反相高效液相色谱分析方法.以VP-ODSC18(5μ)(150×4.6 mm)为分析柱,流动相为乙腈∶2%
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通过对30只健康鸡单剂量口服和静注二氟沙星,建立了测定不同时间血浆二氟沙星浓度的反相高效液相色谱分析方法.以VP-ODSC18(5μ)(150×4.6 mm)为分析柱,流动相为乙腈∶2%四丁基溴化胺(13∶87,V/V),流速1.5 ml*min-1,紫外检测波长为280 nm,最低检出浓度是0.05 μg/ml,回收率(95±0.6)%.
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