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本研究探讨次声作用后脑皮层组织环核甘酸与花生四烯酸代谢产物血栓素A2 (TXA2 )和前列环素(PGI2 )代谢改变及意义。将 40只SD大鼠随机分为正常对照、次声作用 1次、7次、1 4次及代谢性谷氨酸受体拮抗剂α 甲基 4 羧基苯氨基乙酸 (MCPG)治疗 5组。采用本校研制的次声压力仓。次声作用组用 8Hz、1 2 0dB的次声按规定次数 ,每次作用 2h。各组到预定时间后 ,取脑组织匀浆 ,用放免进行环磷酸腺苷 (cAMP)、环磷酸鸟苷(cGMP)与TXB2 及 6 酮 (TXA2 、PGI2 稳定代谢产物 )含量测定 ,并对脑组织匀浆进行蛋白定量。结果 :cAMP、cGMP与TXB2 及 6 酮含量 ,在次声作用 1次组 ,无显著改变 ;在 7次与 1 4次组 ,cAMP与TXB2 含量显著升高 (P<0 .0 1 ) ,6 酮含量明显降低 (P <0 .0 1 )。TXB2 /6 酮比值呈递增趋势 ;治疗组 :cAMP、TXB2 及 6 酮含量与TXA2 /PGI2 比值有明显恢复。提示次声可以通过引起脑cAMP、cGMP与TXA2 、PGI2 代谢改变造成脑损害 ,这是次声导致脑损害的重要因素之一。在相同强度的次声的作用下 ,作用次数的多少与脑cAMP、cGMP与TXA2 、PGI2 代谢改变程度呈直接相关关系。MCPG可能通过影响脑cAMP与TXA2 、PGI2 代谢改变而起脑保护作用。
This study was aimed to investigate the changes of metabolism of thromboxane A2 (TXA2) and prostacyclin (PGI2) in cerebral cortex tissue after infrasound and its significance. Forty Sprague-Dawley rats were randomly divided into normal control, infrasound acupuncture treatment once, seven times, 14 times, and metabolic glutamate receptor antagonist α-methyl-4-carboxy-phenethenylaminoacetic acid (MCPG) Infrasound pressure chamber developed by our school. Infrasound group with 8Hz, 120dB infrasound according to the required number of times, each role 2h. Tissues of each group were sacrificed after a predetermined time, and the content of cAMP, cGMP, TXB2 and 6 ketones (TXA2 and PGI2 stable metabolites) were determined by radioimmunoassay. Tissue homogenate for protein quantification. Results: The contents of cAMP, cGMP, TXB2 and ketone 6 did not change significantly in the first infrasound treatment. The levels of cAMP and TXB2 in the 7 and 14 groups were significantly increased (P <0.01), 6 Ketone content was significantly lower (P <0.01). TXB2 / 6 ketone ratio showed an increasing trend; treatment group: cAMP, TXB2 and 6 ketone content and TXA2 / PGI2 ratio significantly restored. Tip infrasound can cause cerebral cAMP, cGMP and TXA2, PGI2 metabolism caused by brain damage, which is one of the important infrasound causes brain damage. In the same intensity of infrasound, the role of the number of times and brain cAMP, cGMP and TXA2, PGI2 metabolism is directly related to the degree of change. MCPG may play a neuroprotective role by affecting cAMP, TXA2 and PGI2 metabolism.