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目的对实验动物垫料进行微生物指标监测,为管理机构制定垫料的卫生标准提供依据,为垫料生产企业在加工、灭菌、包装、运输、储存等环节及动物使用单位对动物实验结果影响因素分析方面提供参考依据。方法对来自不同实验动物生产和使用单位的垫料,参照GB4789-2010、2012检测方法进行菌落总数、霉菌及大肠杆菌计数、沙门菌的检测。结果 49份未灭菌的垫料存在不同程度的细菌、霉菌及大肠杆菌污染,未检出沙门菌;不同材质的垫料在不同条件下灭菌,在121℃30 min的条件下具有最佳灭菌效果,各项微生物指标均合格;已灭菌的21份垫料有19份存在不同程度的细菌及霉菌污染,未检出沙门菌及大肠杆菌;松软的混合型刨花垫料在灭菌后7 d检测到细菌;采用敞开编织袋储存5 d即检测到细菌污染;垫料使用1 d后的菌量可达104CFU/g,细菌及霉菌含菌量按天数逐步递增,但未检出沙门菌。结论垫料使用前必须消毒灭菌,采用合理的方式储存,及时更换可改善饲养环境,可减少微生物污染,保障实验动物的质量及实验结果的可靠。
OBJECTIVE To monitor the microbial indicators of litter in laboratory animals and provide the basis for the establishment of sanitary standards for litter in the regulatory agencies. It will affect the experimental results of animal experiments in processing, sterilization, packaging, transportation and storage of litter and animal use units Factor analysis to provide a reference. Methods The litter from different experimental animals production and use units, with reference to GB4789-2010,2012 detection methods for the total number of colonies, mold and Escherichia coli count, Salmonella detection. Results 49 non-sterilized litter samples were contaminated by bacteria, mold and Escherichia coli to some extent, and Salmonella was not detected. The litter with different materials was sterilized under different conditions and had the best conditions at 121 ℃ for 30 min Sterilization effect, the indicators of the various microorganisms are qualified; There are 19 21 sterilized litter there are varying degrees of bacterial and fungal contamination, did not detect Salmonella and E. coli; loose mixed-type wood shavings in the sterilization Bacteria were detected on the 7th day. Bacterial contamination was detected after 5 days of storage in open woven bags. The bacterial count reached 104CFU / g after 1day of litter, and the bacteria and molds increased gradually in days but not detected Salmonella. Conclusion The litter must be sterilized before use, stored in a rational way and replaced in time to improve the feeding environment, reduce microbial contamination, and ensure the quality of experimental animals and the reliable experimental results.