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目的:观察D-松醇对慢性哮喘小鼠模型转化生长因子(TGF-β1)的影响。方法:18只BALB/c小鼠按随机原则分成正常对照组、哮喘模型组、D-松醇干预组,每组6只,末次抗原激发24 h后,检测各组小鼠的肺功能,HE染色观察气道炎症变化;Masson三色染色观察气道纤维化的改变;real-time PCR观察肺组织TGF-β1 mRNA的表达;采用ELISA观察支气管肺泡灌洗液中TGF-β1的表达。结果:哮喘模型组小鼠气道炎症、气道高反应性和气道重塑明显加重,而D-松醇能显著抑制慢性哮喘模型小鼠的气道炎症和气道高反应性,且D-松醇干预后哮喘模型小鼠肺组织TGF-β1 mRNA和肺泡灌洗液中TGF-β1表达也显著降低。结论:D-松醇能抑制慢性哮喘模型小鼠的气道重塑,其机制可能通过抑制肺组织TGF-β1的表达而实现。
Objective: To observe the effects of D-pinitol on the expression of transforming growth factor-β1 (TGF-β1) in a mouse model of chronic asthma. Methods: Eighteen BALB / c mice were randomly divided into normal control group, asthma model group and D-pineol intervention group, with 6 rats in each group. After the last antigen challenge for 24 hours, the lung function of each group was detected. HE The changes of airway inflammation were observed by staining. The changes of airway fibrosis were observed by Masson’s trichrome staining. The expression of TGF-β1 mRNA in lung tissue was observed by real-time PCR. The expression of TGF-β1 in bronchoalveolar lavage fluid was detected by ELISA. Results: Airway inflammation, airway hyperresponsiveness and airway remodeling were significantly increased in asthmatic mice, whereas D-pinitol significantly inhibited airway inflammation and airway hyperresponsiveness in mice with chronic asthma, and D-pinene After alcohol interference, the expression of TGF-β1 mRNA in lung tissue and the expression of TGF-β1 in bronchoalveolar lavage fluid of asthmatic mice were significantly decreased. CONCLUSION: D-Pinitol can inhibit the airway remodeling in chronic asthmatic mice, and its mechanism may be through inhibiting the expression of TGF-β1 in lung tissue.