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目的:观察正骨通痹丸对双侧去卵巢所致大鼠骨质疏松的影响,以明确其疗效并探讨其作用机制。方法:使用10周龄Wistar大鼠,采用双侧去卵巢手术的方法建立去卵巢骨质疏松动物模型。模型成立后,假手术组及模型组每天灌胃给予蒸馏水10ml/kg,正骨通痹丸各剂量组、仙灵骨葆胶囊组及雌二醇组每天分别连续灌胃给予正骨通痹丸1.35、2.7、5.4g/kg、仙灵骨葆胶囊0.4g/kg及雌二醇60μg/kg,给药治疗11周后处死,分离子宫;采血,进行ALP和StrACP活性测定以及OC、TNF-α和IL-6含量的测定。结果:与假手术组相比,去卵巢组大鼠血清ALP、StrACP活性及血清OC、TNF-α及IL-6含量均显著升高。正骨通痹丸剂量依赖性地对抗去卵巢所致的血清StrACP及血清ALP活性的增加,且各剂量组OC含量显著下降;正骨通痹丸剂量依赖性地降低大鼠的TNF-α及IL-6含量。结论:正骨通痹丸抗骨质疏松作用机制与其抑制IL-6及TNF-α产生,抑制骨吸收,促进骨形成,维持骨转换平衡有关。
OBJECTIVE: To observe the effect of Zhenggu Tongyu Pill on osteoporosis in rats induced by bilateral ovariectomy to clarify its efficacy and explore its mechanism of action. METHODS: A 10-week-old Wistar rat was used to establish an animal model of ovariectomized osteoporosis using bilateral ovariectomy. After the model was established, the sham operation group and the model group were given distilled water 10ml/kg per day. Each dose of Zhenggu Tongyu Pills, Xianlingguyu capsule group, and estradiol group were administered orally with Zhenggu Tongyu Pills daily for 1.35, respectively. 2.7, 5.4g/kg, Xianlingguyu capsule 0.4g/kg and estradiol 60μg/kg, were sacrificed after 11 weeks of treatment, and the uterus was isolated; blood was taken for ALP and StrACP activity determination and OC, TNF-α and Determination of IL-6 content. RESULTS: Compared with the sham-operated group, serum ALP, StrACP activity and serum OC, TNF-α and IL-6 levels in the ovariectomized rats were significantly increased. Zhenggu Tongyu Pills dose-dependently inhibited the increase of serum StrACP and serum ALP activity caused by ovariectomy, and the OC content in each dose group decreased significantly; Zhenggu Tongyu Pills dose-dependently reduced rats’ TNF-α and IL- 6 content. Conclusion: The anti-osteoporosis mechanism of Zhenggu Tongyu Pill is related to its inhibition of IL-6 and TNF-α production, inhibition of bone resorption, promotion of bone formation, and maintenance of bone turnover balance.