在选定了MS基本培养基的基础上,通过改变NAA与BA两种植物生长调节剂的浓度与添加量,展开了针对“湘林1号”油茶叶片脱分化后得到的愈伤组织或胚状体细胞进行的植株形态再分化试验。试验结果表明:将呈白色、黄白色或浅黄绿色、凹凸不平、质地疏松的愈伤组织块接种在MS+NAA(0.3 mg/L)+BA(2.0 mg/L)+蔗糖(30 g/L)+琼脂(7 g/L)培养基中30 d后,在白色突起部位分化出了丛生芽且生长良好。利用油茶的再生芽接种时,其增殖系数为6.50。 Based on the selection of basic MS medium and the change of concentration and amount of NAA and BA plant growth regulators, we conducted a series of experiments on the callus obtained after dedifferentiation of “Xianglin 1” Or embryoid somatic cell morphological re-differentiation test. The results showed that the callus pieces with white, yellowish white or light yellowish green, irregular texture and loose texture were inoculated into MS + NAA (0.3 mg / L) + BA (2.0 mg / L) ) + Agar (7 g / L) medium for 30 days, clumps budded and grew well on the white protuberances. When regenerating buds of Camellia oleifera were used for inoculation, the proliferation coefficient was 6.50.