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目的探讨Wnt/β-catenin信号通路对孤独症发生过程中重复呆板样行为的影响。方法利用丙戊酸(valproic acid,VPA)孤独症动物模型,检测了经典Wnt信号通路关键信号分子β-catenin及其负性调节因子GSK-3β在孤独症模型大鼠小脑脑区的表达变化;同时检测孤独症模型大鼠重复呆板样行为变化。Western blotting法检测GSK-3β、β-catenin总蛋白及磷酸化蛋白表达,运用旷场实验检测重复呆板样行为持续的时间、次数。结果与对照组相比,在小脑脑区模型组GSK-3β磷酸化蛋白表达增加,β-catenin磷酸化蛋白表达减少;重复呆板样行为持续的时间、次数均增加。结论孤独症大鼠小脑脑区经典wnt信号通路活性增加,运动亢进,提示经典Wnt信号通路活性增加可能导致重复呆板样行为变化,进而导致对孤独症的易感性增加。
Objective To investigate the effect of Wnt / β-catenin signaling pathway on the repetitive behavior of autism. Methods The autophagy model of valproic acid (VPA) was used to detect the expression of β-catenin and its negative regulator GSK-3β in the cerebellum brain of autism model rats. Simultaneous detection of autism in rats repeated stereotyped behavior changes. The total protein and phosphorylated protein of GSK-3β and β-catenin were detected by Western blotting. The open-field test was used to detect the duration and frequency of repetitive dumb-like behavior. Results Compared with the control group, the expression of phosphorylated GSK-3β in the cerebellum brain model group was increased and the expression of β-catenin phosphorylated protein was decreased. The duration and number of repetitive dumb-like behaviors were increased. Conclusions The activity of canonical wnt signaling pathway in cerebellum of autistic rats is increased and hyperactivity suggests that the increase of the activity of canonical Wnt signaling pathway may lead to the change of repetitive dumb-like behavior, leading to the increased susceptibility to autism.