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[目的]观察细胞分化剂喜滴克对肝癌相关基因及血清AFP水平的调节。[方法]人移植性高转移肝癌裸鼠模型LCI D20,随机分为对照组(C组) ,喜滴克低剂量组(L组) ,喜滴克中剂量组(M组) ,喜滴克高剂量组(H组)和喜滴克高剂量 +5 Fu组(H +CT组)。接种后第11天给药 ,连续20天 ,计算抑瘤率 ,测定血清AFP水平和观察腹腔转移。应用RT PCR方法 ,研究基因的表达。应用Western印迹检测整合素β1和E 钙粘素的表达 ,观测高转移人肝癌细胞株MHCC97对纤维连接蛋白的粘附抑制作用。[结果]不同剂量组的喜滴克均呈现一定的抑瘤作用。喜滴克治疗组血清AFP水平比对照组有明显下降 ;对原癌基因c myc、N ras、c jun、c fos及mmp 9基因的表达有明显的下调作用(P<0.01)。喜滴克亦能下调整合素β1蛋白表达和增加E 钙粘素的表达 ;有抑制高转移人肝癌细胞株MHCC97对纤维连接蛋白粘附的能力。[结论]喜滴克对肝癌相关基因及AFP表达的调节可能是其抑制肿瘤生长和转移的分子基础。
[Objective] To observe the regulation of hepatocellular carcinoma related gene and serum AFP by cell differentiation agent Xitiao Ke. [Method] The LCI D20 model of human hepatocellular carcinoma in nude mice was randomly divided into three groups: control group (C), low-dose Xitiaoke group (L), middle-dose Xitiaoke group High dose group (H group) and hi drop grams high dose +5 Fu group (H + CT group). On the 11th day after inoculation, the mice were dosed for 20 consecutive days. The tumor inhibition rate, serum AFP level and abdominal cavity metastasis were observed. RT PCR method was used to study the gene expression. The expression of integrin β1 and E-cadherin was detected by Western blotting, and the inhibitory effect of high metastatic human hepatoma cell line MHCC97 on fibronectin adhesion was observed. [Results] XiDiK in different dose groups all showed certain anti-tumor effect. The serum AFP level of Xitiaoke treatment group was significantly lower than that of the control group. The expression of c-myc, N ras, c jun, c fos and mmp 9 genes was significantly down-regulated (P <0.01). Xi drop grams also down regulate integrin β1 protein expression and increase the expression of E cadherin; inhibit the high metastasis of human hepatoma cell line MHCC97 on fibronectin adhesion. [Conclusion] Xitiaoke regulation of liver cancer related genes and AFP expression may be the molecular basis of its inhibition of tumor growth and metastasis.