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目的利用动物模型观察实验型阻塞型睡眠呼吸暂停综合征小型猪的血浆同型半胱氨酸(Hcy)水平,以探讨睡眠呼吸暂停致动脉粥样硬化的可能机制。方法中国小型猪16头,分为阻塞型睡眠呼吸暂停组(OSA组)和对照组,每组8头。OSA组用凝胶注射法制作动物模型。分别于制作模型前及12周后取实验动物静脉血检测血浆Hcy、叶酸及维生素B12水平。结果(1)造模后,OSA组Hcy水平明显升高,前后分别为(8.94±2.43)和(12.25±1.44)μmol/L,两者相比差异有统计学意义(P≤0.05),对照组Hcy水平无明显变化。造模后OSA组与对照组Hcy值相比差异有统计学意义(P<0.05)。(2)两组动物叶酸及维生素B12水平实验后与实验前相比无明显变化。结论实验性OSA使机体血浆中Hcy水平异常升高,促进机体动脉粥样硬化的发生和发展。
Objective To observe the level of plasma homocysteine (Hcy) in experimental obstructive sleep apnea syndrome (SD) mini-pigs using animal model to explore the possible mechanism of atherosclerosis induced by sleep apnea. Methods Sixteen Chinese miniature pigs were divided into obstructive sleep apnea group (OSA group) and control group (n = 8). OSA group using gel injection animal model. Venous blood samples of experimental animals were used to detect plasma Hcy, folic acid and vitamin B12 levels before and 12 weeks later. Results After modeling, the level of Hcy in OSA group was significantly higher (8.94 ± 2.43) and (12.25 ± 1.44) μmol / L before and after the operation, the difference was statistically significant (P≤0.05) Hcy levels did not change significantly. The difference of Hcy between OSA group and control group after modeling was statistically significant (P <0.05). (2) After two groups of animals folate and vitamin B12 levels experiment no significant change compared with before the experiment. Conclusion The experimental OSA can make the plasma Hcy levels abnormally increase and promote the occurrence and development of atherosclerosis in the body.