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利用固相微萃取法收集施用印楝素对褐飞虱诱导的水稻挥发物,并通过气相色谱-质谱联用仪对其进行分离和鉴定.共收集到褐飞虱为害稻株挥发物25种,其中已定性组分14种,以倍半萜类化合物(9种)为主.褐飞虱为害稻株挥发物比健康稻株多柠檬烯、芳樟醇、水杨酸甲酯、未知6、未知7、姜烯、橙花叔醇、正十七烷8种组分.印楝素处理未导致新的挥发物组分产生,但使褐飞虱诱导的挥发物组分相对含量发生变化.印楝素的浓度导致柠檬烯、芳樟醇、水杨酸甲酯、未知6、姜烯、正十六烷6种组分相对含量发生明显变化;而水杨酸甲酯、未知6、未知7、姜烯、橙花叔醇5种挥发物组分相对含量因施药天数不同而存在显著差异;印楝素与水稻品种、害虫密度等生物因子间的互作使所有虫害诱导挥发物组分相对含量均发生显著改变.
The volatiles of rice plants induced by Nilaparvata lugens (Nilaparvata lugens) were collected by solid phase microextraction (SPME), and were separated and identified by gas chromatography-mass spectrometry (GC-MS) .A total of 25 species of volatiles were collected from rice planthoppers, Fourteen species were sesquiterpenes (nine species), and the volatiles of the brown planthopper (Nilaparvata lugens) were more limonene, linalool, methyl salicylate, unknown 6, unknown 7, Nerolidol and n-heptadecane, while azadirachtin treatment did not result in the production of new volatile components, but changed the relative content of volatile components induced by brown planthopper. The concentration of azadirachtin led to the increase of limonene, Linalool, methyl salicylate, unknown 6, ginger, n-hexadecane six components of the relative content of significant changes; and methyl salicylate, unknown 6, unknown 7, ginger, nerolidol The relative contents of the five volatile components were significantly different due to the different application days. The interaction between azadirachtin and rice varieties, pest density and other biological factors significantly changed the relative contents of all volatile components in the pests.