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以杉木成熟合子胚为起始外植体,研究基本培养基、不同种类的细胞分裂素及生长素对杉木器官发生和体胚发生及不定芽生根的影响,并用石蜡切片法观察不定芽和体胚的发生和发育过程。结果表明:DCR+6_BA1·0mg·L-1+TDZ0·002 mg·L-1+NAA0·1 mg·L-1对成熟合子胚诱导不定芽最有效;DCR+6_BA1·0 mg·L-1+TDZ0·003mg·L-1+ NAA0·1 mg·L-1是成熟合子胚诱导体胚的最佳培养基;不定芽在DCR基本培养基中可有效伸长;1/4MS+IBA0·2 mg·L-1+NAA0·1 mg·L-1对不定芽生根最有效。石蜡切片观察表明:不定芽有2种发生方式,一种是表皮及表皮下的3 ~4层细胞分生组织化发育而成,另一种是外植体内部的小维管束脱分化发育而成。2种发生方式均为直接器官发生途径。
In order to study the effects of basic medium, different kinds of cytokinins and auxins on the development of organ and somatic embryogenesis and adventitious buds in Chinese fir, the mature explants of Cunninghamia lanceolata were used as explants. The adventitious buds and bodies Embryogenesis and development. The results showed that DCR + 6_BA1 · 0 mg · L-1 + TDZ0 · 002 mg · L-1 + NAA0 · 1 mg · L-1 was most effective for inducing adventitious buds in mature zygotic embryos; DCR + 6_BA1 · 0 mg · L- + TDZ0 · 003mg · L-1 + NAA0 · 1 mg · L-1 is the best medium for inducing somatic embryos in mature zygotic embryos; Adventitious buds can be effectively elongated in DCR minimal medium; 1 / 4MS + IBA0 · 2 mg · L-1 + NAA0 · 1 mg · L-1 was most effective for rooting of adventitious buds. Paraffin section observation showed that there are two kinds of adventitious buds occur, one is epidermal and epidermal cells 3 to 4 layers of meristematic development, and the other is the explant within the small vascular bundle dedifferentiation development to make. Two kinds of occurrence are direct organ pathways.