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目的 :高效液相色谱法测定参芍片中人参皂苷Rg1,Re 和芍药苷的含量。方法 :SymmetryC18柱。流动相 :乙腈 0 .2 %磷酸 (11∶89) ,流速 :0 .8mL/min ,柱温 :30°C ,检测波长 :2 0 3,2 30nm ,进样量 10 μL。 结果 :本法可测定人参皂苷Rg1,Re 和芍药苷的含量 ,其分别在 0 .96 0 μg～ 4.80 0 μg ;0 .912 μg～ 4.5 6 0 μg ;1.0 5 0 μg～5 .2 5 0 μg范围内与峰面积成线性关系。平均回收率分别为 10 5 .2 % ;98.5 % ;10 3.5 %。结论 :该方法简便、准确 ,可作为参芍片的含量测定方法
Objective: To determine the contents of ginsenoside Rg1, Re and paeoniflorin in Shenqi Tablets by HPLC. Method: SymmetryC18 column. Mobile phase: acetonitrile 0.2% phosphoric acid (11:89), flow rate: 0.8 mL/min, column temperature: 30°C, detection wavelength: 2 0 3, 2 30 nm, and injection volume 10 μL. Results: The method can determine the content of ginsenosides Rg1, Re and paeoniflorin, which are in the range of 0. 960 0 μg to 4.80 0 μg; 0 .912 μg to 4.5 600 μg; 1.0 50 μg to 5 . The linear relationship between the μg range and the peak area. The average recoveries were 105.9%; 98.5%; 103.5%. Conclusion : This method is simple and accurate. It can be used as a method for determining the content of Shenqi tablets.