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目的 探讨外周血单核细胞及其雌激素受体 ( ER) m RNA表达水平 ,在绝经后骨质疏松发生机理中所起的作用。方法 成年雌性 SD大鼠 30只 ,随机分 3组 ,去势组 ( OVX组 ) ,假手术组 ( Sham组 ) ,OVX+Premarin组 ( ERT组 )。术后 6周后采各大鼠心脏血 ,分别以 1用放免法测定血清雌二醇 ;2外周血单核细胞计数以及分离培养纯化单核细胞后运用 RT- PCR法检测 ER- m RNA;3各组行骨组织形态计量学测定。结果 1OVX组与 Sham组相比 ,血清 E2 显著下降 ( P<0 .0 1)。外周血单核细胞( PBM)数显著升高 ( P<0 .0 1) ,而 PBM的 ER- m RNA表达显著降低 ( P<0 .0 1) ,骨组织形态计量学检查 OVX组显示骨小梁面积 ,骨小梁宽度 ,皮质骨面积 ,骨小梁游离末端数均明显降低 ( P<0 .0 5) ,而骨小梁表面破骨细胞数 ,骨矿化沉积率 ,骨矿化缘长度及骨小梁间距等都显著增加 ( P<0 .0 5,P<0 .0 1) ,2 ERT组的血清 E2 ,PBM数及其 ER- m RNA与 Sham组相比无显著差异 ( P<0 .0 5) ,骨量及显微结构亦无明显减少和破坏 ( P<0 .0 5) ,但与 OVX组相比 ,则各项指标均有显著差异 ( P<0 .0 5)。结论 1卵巢切除后骨量丢失可能与单核细胞表达 ER量减少 ,使单核细胞向破骨细胞的分化增多 ,而使骨吸收增强有关。 2激素替代治疗可能通过调节单核?
Objective To explore the role of mRNA expression of peripheral blood mononuclear cells and its receptor estrogen receptor (ER) in the pathogenesis of postmenopausal osteoporosis. Methods Thirty adult female Sprague-Dawley rats were randomly divided into 3 groups: OVX group, sham operation group and OVX + Premarin group (ERT group). Six weeks after the operation, all the rat’s heart blood was collected and the levels of serum estradiol were determined by radioimmunoassay (RIA) .2 Peripheral blood mononuclear cells (PBMCs) were counted and the monocytes were cultured and purified. RT-PCR was used to detect ER- Group bone histomorphometry measurement. Results Compared with Sham group, serum E2 decreased significantly in 1OVX group (P <0.01). The number of peripheral blood mononuclear cells (PBM) was significantly increased (P <0.01), while the expression of ER-m RNA in PBM was significantly decreased (P <0.01). The bone histomorphometry of OVX group Trabecular area, trabecular width, cortical bone area and trabecular free ends were significantly decreased (P <0.05), while the number of trabecular bone osteoclast, bone mineral deposition rate, bone mineralization margin (P <0.05, P <0.01). The levels of E2, PBM and ER-mRNA in ERT group were not significantly different from those in Sham group (P <0.05, P < P <0. 05), there was no significant reduction and destruction of bone mass and microstructure (P <0.05), but all the indexes were significantly different compared with OVX group (P <0. 0 5) 5). Conclusion 1 The amount of bone loss after ovariectomy may be related to the decrease of ER expression in monocytes, the increase of mononuclear cells to osteoclasts and the increase of bone resorption. 2 hormone replacement therapy may regulate mononuclear?