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目的:分析、掌握北京市宣武区流感病原学变化特点及流行趋势,为该地区流感的防治提供科学依据。方法:采集流感病例(ILI)的咽拭子标本用狗肾细胞(MDCK)进行病毒分离培养,采用血凝(HA)及血凝抑制(HI)方法进行流感病毒初筛及分型鉴定。结果:2009年共采集标本1426份,分离出流感病毒127株,分离率8.90%,流感流行优势株A(H3N2)亚型为79株;2010年共采集标本1966份,分离出流感病毒192株,分离率9.77%,流感流行优势株为A(H3N2)亚型84株和B(Victoria)型79株。结论:2009年和2010年宣武区的流感病毒优势株在发生变化,且流感流行季也在发生变迁,新甲H1N1型已作为常态监测6,0岁以上人群的病毒分离率增加,应加强监测,密切注意流行株的活动情况。
OBJECTIVE: To analyze and grasp the characteristics and trends of influenza etiology in Xuanwu District of Beijing, and provide a scientific basis for the prevention and control of influenza in the area. Methods: The throat swab specimens from influenza cases (ILI) were collected for virus isolation and culture using dog kidney cells (MDCK). The primary screening and typing of influenza virus were performed by hemagglutination (HA) and hemagglutination inhibition (HI) methods. Results: A total of 1426 samples were collected in 2009, of which 127 isolates were isolated, the isolation rate was 8.90%. The influenza A (H3N2) subtype was 79 strains. A total of 1966 specimens were collected in 2010, and 192 strains of influenza virus were isolated , The isolation rate was 9.77%. The predominant influenza strains were 84 strains of A (H3N2) subtype and 79 strains of B (Victoria) strain. Conclusion: The predominant strains of influenza virus in Xuanwu District in 2009 and 2010 are changing, and the epidemic season of influenza is also changing. The new H1N1 type has been used as a routine measure to monitor the increase of the virus isolation rate in people over 6 years of age. Monitoring should be strengthened , Pay close attention to the activities of epidemic strains.