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目的:探讨环维黄杨星D(Cvb-D)对甲状腺素诱导大鼠心肌肥厚的保护作用及机制。方法:除正常对照组外,各组每日均腹腔注射左旋甲状腺素(L-Thy)1 mg.kg-1,连续14 d,制备大鼠心肌肥厚模型。末次给药24 h后处死大鼠,计算大鼠心脏质量指数(全心质量/体质量,HW/BW)和左心室质量指数(左心室质量/体质量,LVW/BW),观察心肌组织病理形态学改变,并测定心肌组织中一氧化氮(NO)、一氧化氮合成酶(NOS)、超氧化物歧化酶(SOD)、丙二醛(MDA)的含量,评价Cvb-D对大鼠心肌肥厚的影响及可能机制。结果:与对照组比较,L-Thy模型组大鼠心脏质量指数明显升高,病理切片可见心肌肥厚改变,心肌NO、NOS和SOD含量显著降低,MDA显著增加。与模型组相比,Cvb-D中、高剂量组(12,24 mg.kg-1)均能明显降低小鼠HW/BW和LVW/BW,病理切片可见损害较模型组减轻,心肌NO、NOS、SOD含量显著升高(P<0.05),MDA含量显著下降(P<0.01)。结论:环维黄杨星D对L-Thy诱导的大鼠心肌肥厚具有一定的保护作用,其作用机制可能与其抗心肌氧化损伤有关。
Objective: To investigate the protective effect and mechanism of Cvb-D on thyroxin-induced cardiac hypertrophy in rats. Methods: Except normal control group, L-Thy (1 mg.kg-1) was intraperitoneally injected into each group for 14 consecutive days. The rats were sacrificed 24 h after the last administration, and the cardiac mass index (total heart mass / body weight, HW / BW) and left ventricular mass index (LV mass / body weight, LVW / BW) Morphological changes were observed and the content of nitric oxide (NO), nitric oxide synthase (NOS), superoxide dismutase (SOD) and malondialdehyde (MDA) in myocardial tissue were measured to evaluate the effect of Cvb- Effect of cardiac hypertrophy and possible mechanism. Results: Compared with the control group, the cardiac mass index of L-Thy model group was significantly increased. The changes of myocardial hypertrophy and the content of NO, NOS and SOD in myocardium were significantly decreased and MDA significantly increased. Compared with the model group, Cvb-D medium and high dose groups (12, 24 mg.kg-1) can significantly reduce the mice HW / BW and LVW / BW, pathological sections showed lesion damage than the model group, myocardial NO, NOS, SOD content increased significantly (P <0.05), MDA content decreased significantly (P <0.01). CONCLUSION: Cyclovirobuxine D has a protective effect on L-Thy-induced myocardial hypertrophy in rats, and its mechanism may be related to its anti-myocardial oxidative damage.