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目的探讨建立新生期BALB/c小鼠非致死性肺炎链球菌感染模型的方法。方法将新生期BALB/c小鼠随机分为肺炎链球菌感染1次组(于新生期第7天分别经鼻腔滴注肺炎链球菌D39株107、108、109、1010CFU菌液各10μl)、感染2次组(于新生期第6、7天连续2 d经鼻腔滴注肺炎链球菌D39株107、108、109、1010CFU菌液各10μl)和对照1、2组(于第7天和第6、7天分别经鼻腔滴注PBS液10μl),每组20只。最后1次鼻腔滴注后24 h随机抽取10只小鼠处死,取肺组织匀浆,进行细菌培养及鉴定,HE染色观察肺组织病理改变。剩余10只观察感染后5 d的体重变化及存活情况。结果感染2次109CFU肺炎链球菌组小鼠出现安静少动,皮肤苍白,体重增长下降;感染率与存活率均>85%;病理显示肺部出现炎症改变,肺泡间隔增厚,血管周围和支气管周围有一定程度的炎症细胞浸润,以中性粒细胞为主。结论新生期第6、7天感染109CFU肺炎链球菌可能是成功建立新生期BALB/c小鼠非致死性肺炎链球菌感染模型的较好方法。
Objective To explore a method for establishing non-lethal S. pneumoniae infection model in neonatal BALB / c mice. Methods Neonatal BALB / c mice were randomly divided into 1 group with streptococcus pneumoniae infection (10μl of 107, 108, 109, and 1010CFU inoculated with S. pneumoniae D39 strain respectively on the 7th day of neonatal period) 2 groups (10μl of 107,108,109,1010CFU bacterial suspension of D39 strain of Streptococcus pneumoniae were instilled intranasally on the 6th and 7th day of the newborn period respectively) and the control group 1 and 2 (on the 7th and the 6th day , 7 days were intranasal instillation of PBS solution 10μl), each group of 20. Twenty-four hours after the last intranasal instillation, 10 mice were randomly selected and killed. Pulmonary tissue homogenate was taken for bacterial culture and identification. Pathological changes of lung tissue were observed by HE staining. The remaining 10 animals were observed after 5 days of weight changes and survival. Results In mice infected with 109 CFU of Streptococcus pneumoniae, the animals were sedentary and sedentary, with pale skin and decreased body weight. The infection rate and survival rate were both higher than 85%. The pathological findings showed inflammatory changes in the lungs, thickening of the alveolar septa, perivascular and bronchial Around a certain degree of inflammatory cell infiltration, mainly to neutrophils. Conclusions Infection of 109 CFU of Streptococcus pneumoniae on the 6th and 7th day of newborn period may be a good method to establish a non-lethal S. pneumoniae infection model in neonatal BALB / c mice.