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[Objective] This study aimed to construct DNA fingerprint for hybrid rice cultivars those have been approved by Hunan Province. [Method] The primers which produced polymorphic and bright DNA bands were selected to construct the DNA molecular fingerprint map for 77 major hybrid rice cultivars approved by Hunan Province. [Result] A total of 48 SSR primers were selected. Every cultivar had its unique fingerprint map so that the obtained data could identify different hybrid rice cultivars. [Conclusion] This study made great contributions to the perfection of hybrid rice germplasm identification.
[Objective] This study aimed to construct DNA fingerprint for hybrid rice cultivars those have been approved by Hunan Province. [Method] The primers which produced polymorphic and bright DNA bands were selected to construct the DNA molecular fingerprint map for 77 major hybrid rice cultivars approved By Hunan Province. [Result] A total of 48 SSR primers were selected. Every cultivar had its unique fingerprint map so that the the data obtained could have identified different hybrid rice cultivars. [Conclusion] This study made great contributions to the perfection of hybrid rice germplasm identification.