论文部分内容阅读
目的:通过建立成熟的SYBR Green I实时荧光定量PCR检测血浆中mi R-155的表达,并探讨mi RNAs与乳腺癌的关系。方法:选取符合纳入标准的41例乳腺癌患者作为研究对象、25例乳腺良性疾病(包括乳腺囊性增生病、乳房纤维腺瘤和乳管内乳头状瘤)患者作为良性对照组、38例健康体检者作为健康对照组,采用茎环引物的SYBR Green I实时荧光定量PCR检测外周血浆中mi R-155的含量。结果:SYBR Green I实时荧光定量PCR能特异性检测血浆中mi RNA-155的扩增信号,熔解曲线峰值单一,产物特异;乳腺癌患者血清中mi RNA-155水平明显高于乳腺良性疾病(P<0.05),而乳腺癌患者手术切除后血清中的mi RNA-155水平明显下降(P<0.05)。结论:SYBR Green I实时荧光定量PCR法具有灵敏度高、操作简单且成本低廉等特点,利用此法检测mi R-155的表达,对乳腺癌的早期诊断和预后判断有很好的应用前景。
OBJECTIVE: To detect the expression of mi R-155 in plasma by real-time quantitative PCR with SYBR Green I and explore the relationship between mi RNAs and breast cancer. Methods: Forty-one patients with breast cancer who met the inclusion criteria were enrolled in the study. Twenty-five patients with benign breast diseases (including cystic mastocystosis, breast fibroadenoma and intraductal papilloma) were selected as the benign control group. Thirty-eight healthy subjects As a healthy control group, the content of mi R-155 in peripheral plasma was detected by SYBR Green I real-time PCR using stem-loop primers. Results: SYBR Green I real-time quantitative PCR could detect the miRNA-155 signal in plasma. The peak of melting curve was single and the product was specific. The level of mi RNA-155 in breast cancer patients was significantly higher than that in benign breast diseases <0.05), while the level of mi RNA-155 in the serum of patients with breast cancer was significantly decreased (P <0.05). Conclusion: SYBR Green I real-time fluorescence quantitative PCR method has the characteristics of high sensitivity, simple operation and low cost. Using this method to detect the expression of mi R-155, it has a good prospect for early diagnosis and prognosis of breast cancer.