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目的探讨17β-雌二醇(17β-estradiol,E2)对人甲状腺滤泡状癌WRO细胞侵袭能力的影响及其机制。方法用10-8mol/L的E2处理WRO细胞不同时间(0、24、48 h),倒置显微镜下观察细胞形态的变化;RT-PCR和Western blot法检测细胞中G蛋白偶联雌激素受体(G protein-coupled estrogen receptor,GPER)、CXCR1、基质金属蛋白酶2(matrix metalloproteinase2,MMP2)和MMP9基因mRNA的转录水平和蛋白的表达水平;ELISA法检测细胞中IL-8的含量;Transwell小室法检测细胞的侵袭能力。结果随着E2处理时间的延长,WRO细胞间的间隙变大,逐渐离散成单个细胞,形态呈长梭形、纺锤形;细胞中GPER、CXCR1、MMP2和MMP9基因mRNA的转录水平和蛋白的表达水平及分泌IL-8的水平均逐渐升高(P<0.05或P<0.01);细胞的侵袭能力明显增强(P<0.05)。结论 E2可通过上调GPER、CXCR1、MMP2、MMP9和IL-8的表达,促进WRO细胞的侵袭。
Objective To investigate the effect of 17β-estradiol (E2) on invasion of human thyroid follicular carcinoma cell line WRO and its mechanism. Methods WRO cells were treated with E2 at 10-8 mol / L for different time (0, 24, 48 h), and the morphological changes were observed under inverted microscope. The expression of G protein-coupled estrogen receptor (GPER), CXCR1, MMP2 and MMP9 mRNA and protein expression levels were measured by enzyme-linked immunosorbent assay (ELISA); Transwell chamber assay Detection of cell invasiveness. Results With the prolongation of E2 treatment, the interspaces of WRO cells became larger and gradually separated into single cells with long fusiform and spindle shapes. The transcription and expression of GPER, CXCR1, MMP2 and MMP9 mRNA (P <0.05 or P <0.01). The invasive ability of cells was significantly enhanced (P <0.05). Conclusion E2 can promote the invasion of WRO cells by up-regulating the expression of GPER, CXCR1, MMP2, MMP9 and IL-8.