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以玉米自交系吉846和掖3189为亲本,组建含有280个F7家系的RILs群体为试验材料,构建含117个SSR位点和50个AFLP位点的遗传连锁图谱,图谱总长度2 638.3cM,标记间平均距离15.8cM。采用复合区间作图法对10个产量及相关性状进行QTL分析,共检测到108个QTL,其中4个QTL相对稳定表达。结果表明,第1染色体上控制株高的qPh-2-1,位于标记P66M89315-P37M70169之间,可提供的表型贡献率为5.72%～8.40%;第3染色体上控制穗位高的qEh-3-1,位于标记P66M47273-umc1400之间,可提供的表型贡献率为17.01%～21.06%,为主效QTL;第4染色体上控制穗长的qEl-4-2,位于标记umc1058-umc2289之间,可提供的表型贡献率为4.42%～6.72%;第8染色体上控制穗粗的qEd-8-1,位于标记bnlg240-umc1268之间,可提供的表型贡献率为5.33%～9.57%。Bin2.00-2.01、Bin2.04-2.05、Bin3.05-3.09、Bin7.01-7.02和Bin8.05-8.07这5个区域可能是产量及相关性状QTL的密集区域。
The RILs population containing 280 F7 lines was constructed from the maize inbred line Ji 846 and Ye 3189, and the genetic linkage map was constructed with 117 SSR loci and 50 AFLP loci, with a total length of 2638.3 cM , The average distance between markers 15.8cM. A total of 108 QTLs were detected in 10 QTLs for yield and related traits using compound interval mapping. Among them, 4 QTLs were relatively stable. The results showed that the phenotypic contribution of qPh-2-1, located on the marker P66M89315-P37M70169, on chromosome 1 was 5.72% -8.40%. The qEh- 3-1, located in the marker P66M47273-umc1400 between the phenotypic contribution rate of 17.01% ~ 21.06%, as the main effect of QTL; on chromosome 4 control ear length qEl-4-2, located in the marker umc1058-umc2289 , The available phenotypic contribution rate was 4.42% ~ 6.72%. On the 8th chromosome, qEd-8-1 with controlled ear diameter was located between the markers bnlg240-umc1268 and the phenotypic contribution rate was 5.33% 9.57%. The five regions Bin2.00-2.01, Bin2.04-2.05, Bin3.05-3.09, Bin7.01-7.02 and Bin8.05-8.07 may be the dense regions of QTL for yield and related traits.