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应用原位杂交和免疫组织化学方法,对成年大鼠延髓内含前-原脑啡肽(PPE)mRNA和甲硫氨酸-脑啡肽(M-ENK)与亮氨酸-脑啡肽(L-ENK)免疫反应神经元进行观察。结果表明:含PPEmRNA的神经元胞体,多数分布在孤束核、腹外侧区以及两者之间的网状结构等形成的一条从背内侧到腹外侧区的弧形带内。M-ENK与L-ENK样免疫反应阳性结构(神经元胞体、纤维和终末)也主要密集分布在该带内。本结果对ENK(M-ENK和L-ENK)参与延髓内脏功能活动的调控过程,提供了进一步的形态学证据。
In situ hybridization and immunohistochemistry were used to detect the expression of proenkephalin (PPE) mRNA, methionine-enkephalin (M-ENK) and leucine-enkephalin L-ENK) immunoreactive neurons were observed. The results showed that most of the neuronal somatic bodies containing PPE mRNA were distributed in the arcuate zone formed by the nucleus tractus solitarius, the ventral lateral region and the reticular structure between the two. M-ENK and L-ENK-like immunoreactive positive structures (neuronal somatosomes, fibers and terminals) are also mainly densely distributed within this band. This result provides further morphological evidence for the involvement of ENK (M-ENK and L-ENK) in the regulation of medullary visceral functional activity.