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【目的】研究姜黄素对前列腺癌细胞株LNCaP细胞增殖的影响。【方法】化学发光法检测不同浓度的姜黄素处理前列腺癌细胞株LNCaP后前列腺特异抗原(PSA)含量,利用荧光素酶报告基因pGL-3构建含PSA基因5’侧启动子区640 bp DNA的荧光素酶表达载体pGL3-PSA,并将其转染LNCaP细胞,不同浓度姜黄素作用24 h后应用荧光素酶测定系统检测荧光素酶表达活性。用免疫印迹Western-blotting技术检测雄激素受体(AR)的表达。【结果】姜黄素抑制LNCaP细胞培基中PSA蛋白及含PSA启动子的荧光素酶活性的表达;形态学结果显示姜黄素可抑制前列腺癌细胞LNCaP的增殖;Western-blotting技术检测结果显示姜黄素抑制AR的表达,并且对AR表达的抑制程度依赖于姜黄素的浓度。【结论】姜黄素对PSA启动子的影响及PSA蛋白表达的抑制作用是通过抑制雄激素受体(AR)的表达实现的,姜黄素能够抑制前列腺癌细胞LNCaP的增殖。
【Objective】 To study the effect of curcumin on the proliferation of prostate cancer cell line LNCaP. 【Methods】 The chemiluminescence method was used to detect the content of prostate specific antigen (PSA) after different concentration of curcumin treatment of prostate cancer cell line LNCaP. The luciferase reporter gene pGL-3 was used to construct 640 bp DNA containing the 5 ’flanking region of PSA gene The luciferase expression vector pGL3-PSA was transfected into LNCaP cells. Luciferase assay system was used to detect luciferase activity after 24 h treatment with different concentrations of curcumin. The expression of androgen receptor (AR) was detected by Western blotting. [Results] Curcumin inhibited the expression of PSA protein and PSA promoter in LNCaP cell culture medium. The morphological results showed that curcumin inhibited the proliferation of prostate cancer cell LNCaP. The results of Western-blotting showed that curcumin Inhibit the expression of AR, and the degree of inhibition of AR expression depends on the concentration of curcumin. 【Conclusion】 The effect of curcumin on the promoter of PSA and the inhibition of the expression of PSA protein are achieved by inhibiting the expression of androgen receptor (AR). Curcumin can inhibit the proliferation of prostate cancer cell LNCaP.