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由ｐＵＲ５２８构建中间载体ｐＥＨＴ９，再构建超甜定植物表达载体ｐＢＩＴ７。通过直接转化法将ｐＢＩＴ７导入农杆菌，然后利用农杆菌系统转化烟草。经ＰＣＲ、ＰＣＲ－Ｓｏｕｔｈｅｒｎ和Ｓｏｕｔｈｅｒｎ杂交证实，超甜定基因已整合到烟草基因组中。
The intermediate vector pEHT9 was constructed by pUR528 and the superboderm expression vector pBIT7 was constructed. PBIT7 was introduced into Agrobacterium by direct transformation followed by Agrobacterium tumefaciens transformation of tobacco. It was confirmed by PCR, Southern blot and Southern blot that the superadrenin gene was integrated into tobacco genome.