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硝苯啶是治疗心血管疾病的一种广泛使用的药物。我们采用反相高效液相色谱法测定了人血浆中硝苯啶的浓度。在碱性条件下用氯仿萃取1ml血浆,萃取物经洗涤浓缩后进样。以60:40的甲醇——磷酸盐缓冲溶液为流动相,在235nm进行紫外检测。本法灵敏度为5ng/ml,标准曲线的线性可达400ng/ml,十次测定的相对标准偏差为7.1％,在浓度为80ng/ml时的回收率为96.8±8.1％。本法已成功地应用于硝苯啶的临床药物监测。
Nifedipine is a widely used drug for the treatment of cardiovascular disease. We measured the concentration of nifedipine in human plasma using reversed-phase high performance liquid chromatography. 1 ml of plasma was extracted with chloroform under alkaline conditions, and the extract was concentrated by washing and then injected. With 60:40 methanol - phosphate buffer solution as the mobile phase, UV detection at 235nm. The sensitivity of this method is 5 ng / ml, the linearity of the standard curve is 400 ng / ml, the relative standard deviation of the ten determinations is 7.1%, and the recovery is 96.8 ± 8.1% at a concentration of 80 ng / ml. This law has been successfully applied to the monitoring of nifedipine in clinical medicine.