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油菜花叶病毒(Oilseed rape mosaic virus,ORMV)武汉株系(Wh)基因组全序列分析表明,该株系基因组全长6 301nt,与烟草花叶病毒属(Tobamovirus)亚组Ⅲ病毒基因组结构相似,含4个开放阅读框架(open reading frame,ORF),ORF 2与ORF 3有77 nt的重叠区。与该属其他病毒对应ORF的核苷酸及编码蛋白氨基酸序列比对,ORMV-Wh与亚组Ⅲ的ORMV、车前草花叶病毒上海株系(Ribgrass mosaic virus,RMV-Sh)、车前草花叶病毒凤仙花株系(RMV-Imp)和烟草花叶病毒十字花科和大蒜株系(Tobacco mosaic virus,TMV-Cg)一致性最高,均超过95%。4种蛋白的氨基酸序列系统进化树构建将Tobamovirus亚组Ⅲ株系分为3个群,ORMV-Wh归为ORMV代表的株系群。
The complete genome sequence analysis of the genome of oilseed rape mosaic virus (ORMV) in Wuhan showed that the full-length genome of the strain was 6 301nt, which was similar to the genome of the virus of subgroup III of Tobamovirus. There are four open reading frames (ORFs), and ORF 2 and ORF 3 have 77 nt overlapping regions. ORMV-Wh was compared with ORMV of subgroup Ⅲ, Ribgrass mosaic virus (RMV-Sh), plantain flower The highest identities of the leaf-spring Impatiens (RMV-Imp) and Tobacco mosaic virus (TMV-Cg) strains were over 95%, respectively. Phylogenetic tree construction of four kinds of proteins The Tobamovirus subgroup III strains were divided into three groups, and ORMV-Wh was classified as a group of strains represented by ORMV.