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目的:研究土茯苓总黄酮(TFSG)对急性痛风性关节炎小鼠的抗炎作用,并初步探讨其作用机制。方法:将90只雄性C57BL/6小鼠随机分为正常组,模型组,秋水仙碱组(0.65 mg·kg~(-1)),土茯苓总黄酮低,中,高剂量组(100,300,500mg-kg~(-1))。,每组15只。除正常组外,其余各组采用尿酸钠结晶复制小鼠痛风性关节炎模型,测定小鼠踝关节肿胀度;酶联免疫吸附法(ELISA)检测不同组别滑膜组织中內细胞介素-1β(IL-1β),IL-6,肿瘤坏死因子-α(TNF-α)水平,蛋白免疫印迹法(Western blot)测定滑膜组织中核苷酸结合寡聚化结构域样受体蛋白3(NLRP3),凋亡相关斑点样蛋白(ASC),半胱氨酸天冬氨酸蛋白酶-1(Caspase-1)的蛋白表达,实时荧光定量PCR(Real-time PCR)检测N LRP3,ASC,Caspase-1 mRNA水平的表达。结果:与正常组比较,模型组小鼠踝关节肿胀度明显增加(P<0.01),模型组小鼠滑膜组织中lL-lβ,IL-6,TNF-α水平显著升高(P<0.01),NLRP3,ASC,Caspase-1蛋白表达和mftNA水平显著升高(P<(h 01);与模型组比较,土茯苓总黄酮组能够显著降低小鼠踝关节肿胀度(PC0.01),滑膜组织中lL-lβ,IL-6,TNF-α水平以及NLRP3,ASC,Caspase-1蛋白水平和mRNA水平显著降低(P<0.01)。结论:土茯苓总黄酮具有一定的抗痛风性关节炎作用,其作用机制与NLRP3炎性体轴有关。
OBJECTIVE: To study the anti-inflammatory effect of TFSG on acute gouty arthritis mice and to explore its mechanism. Methods: Ninety male C57BL / 6 mice were randomly divided into normal group, model group, colchicine group (0.65 mg · kg -1), low, medium and high dose of Smilax glabra total flavonoids (100, 300, 500 mg -kg ~ (-1)). , Each group of 15. In addition to the normal group, sodium urate crystals were used to replicate the models of gouty arthritis in mice in each group, and the degree of ankle swelling in mice was measured. ELISA was used to detect the expression of interleukin- The levels of IL-1β, IL-6 and TNF-α in synovial tissue were determined by Western blotting. NLRP3, ASC and Caspase-1 were detected by real-time PCR, and NRPV, ASC and Caspase were detected by real-time PCR -1 mRNA expression. Results: Compared with the normal group, the degree of ankle swelling in the model group was significantly increased (P <0.01), the levels of IL-1β, IL-6 and TNF-α in the model group were significantly increased ), NLRP3, ASC, Caspase-1 protein expression and mftNA significantly increased (P <(h 01); compared with the model group, Tuckahoe total flavonoids group can significantly reduce mouse ankle swelling (PC0.01) The level of IL-1β, IL-6 and TNF-α and the levels of NLRP3, ASC, Caspase-1 protein and mRNA in synovial membrane were significantly decreased (P <0.01) .Conclusion: Total flavonoids of Smilax glabra with some resistance to gout The role of inflammation, its mechanism of action and NLRP3 inflammatory body axis.