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目的:探讨夏佛塔苷对高脂饮食诱导的小鼠非酒精性脂肪肝的(NAFLD)保护作用。方法:72只雄性C57BL/6小鼠分成6组:正常对照组,模型组,熊去氧胆酸组(60mg/kg),夏佛塔苷低、中、高剂量组(20、40、80mg/kg),灌胃给药8周,除正常对照组外均采用高脂饲料饲养建立NAFLD模型。检测血清和肝组织相关生化指标;观察肝脏组织形态;运用q RT-PCR检测肝组织中Nrf2、HO-1 m RNA的表达。结果:与模型组比较,夏佛塔苷高剂量能够显著降低肝组织MDA含量而升高SOD的含量(P<0.01),提高肝组织中Nrf2、HO-1 m RNA的表达(P<0.01,P<0.05),显著减轻肝脏脂肪变性和炎细胞浸润。结论:夏佛塔苷对高脂饮食诱导的NAFLD有保护作用,可能通过Nrf2/HO-1途径降低组织活性氧的水平,进而减轻脂肪性导致肝损伤。
Objective: To investigate the protective effect of clomattazin on nonalcoholic fatty liver disease (NAFLD) induced by high fat diet in mice. Methods: Seventy-two male C57BL / 6 mice were divided into 6 groups: normal control group, model group, ursodeoxycholic acid group (60mg / kg), low- / kg) for 8 weeks, except for the normal control group were fed with high-fat diet to establish NAFLD model. The biochemical indexes of serum and liver tissue were detected. The morphology of liver tissue was observed. The expression of Nrf2 and HO-1 mRNA in liver tissues was detected by q RT-PCR. Results: Compared with the model group, high-dose of cisplatin significantly reduced the content of MDA and increased the content of SOD in liver tissue (P <0.01), and increased the expression of Nrf2 and HO-1 mRNA (P <0.01, P <0.05), significantly reduce hepatic steatosis and inflammatory cell infiltration. CONCLUSIONS: Clotifoside can protect NAFLD induced by high-fat diet and reduce the level of reactive oxygen species (ROS) through Nrf2 / HO-1 pathway, thereby reducing the risk of fatty liver injury.