Cloning of GF14 Gene from the Bulbs of Fritillaria crrishoa

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GF14 widely occurs in plant species functioning as a regulatory prote in in cell signal transdution, including monoamine synthesis,exocytosis and cell cycle regulation.A partial sequence,413 bp in length,is cloned from the bu lbs of F. crrihosa by means of RT-PCR technique. Se-quence alignment with the BLAST program shows that it is highly similar to GF14 gene. A new full length c DNA,1133 bp,is obtained by RACE techniques.It contains a major open reading f rame of 262 amino acids,5'and 3'untranslated regions and along Poly (A) tail. Sequence analysis of deduced GF14 protein from F.crrihosa indicates several conserved motifs of GF14 proteins are also found in sequence of GF14 protein of F. crrihosa.
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