论文部分内容阅读
目的分析2003-2014年宁波市乙型流行性感冒(流感)病毒血凝素(HA)和神经氨酸酶(NA)的分子流行病学特征。方法采集宁波市2003-2014年哨点监测医院的流感样病例标本进行流感病毒分离鉴定,选取不同时期的乙型流感毒株进行HA和NA基因序列测定,采用生物信息学软件分析变异和进化。结果宁波市乙型流感病毒Victoria和Yamagata两大普系流行株的HA及NA基因在2003-2014年间每年均有不同程度的氨基酸序列变异,其中最明显的为2005年与2009年的Victoria系毒株及2007年与2010年的Yamagata毒株变异。每当HA基因序列发生明显变异时NA基因也同时出现明显的变异,而且有时NA基因的变异比HA基因更明显。同时发现2013-2014年流行的乙型流感病毒为Yamagata系HA与Victoria系NA的重配株。结论乙型流感两大谱系流行株的HA基因发生变异时NA基因也会出现变异,甚至较HA基因更快。2013-2014年宁波市乙型流感高发的流行株为Yamagata系HA与Victoria系NA基因的重配株。
Objective To analyze the molecular epidemiological characteristics of hemagglutinin (HA) and neuraminidase (NA) of influenza B virus in Ningbo City from 2003 to 2014. Methods Samples of influenza-like illness from the sentinel surveillance hospital in Ningbo City during 2003-2014 were collected for influenza virus isolation and identification. Sequence analysis of HA and NA genes of influenza B isolates from different periods were carried out. Bioinformatics software was used to analyze the variation and evolution. Results The HA and NA genes of two universal strains of influenza B and Victoria in Yamagata of Ningbo had varying degrees of amino acid sequence variation every year from 2003 to 2014. Among them, the most obvious were the strains from Victoria in 2005 and 2009 Strain of Yamagata strain in 2007 and 2010. NA gene appears obvious variation at the same time whenever the HA gene sequence has obvious variation, and sometimes NA gene mutation is more obvious than HA gene. At the same time, it was found that the influenza B virus prevailing between 2013 and 2014 was a reassortant of Yama strain of HA and Victoria strain of NA. Conclusion The HA genes of the two major lineage strains of influenza B are mutated and NA genes will be mutated even faster than HA genes. In 2013-2014, the epidemic strain of influenza B in Ningbo City was a reassortment of NA genes of Yamagata strains HA and Victoria strains.