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对窄叶,无叶舌基因杂合(naltnal.lg~+lg)的水稻(Oryza.sativa L.2n=24)杂种经花药培养所诱导的单倍体和二倍体植株进行了遗传分析。在表现显性性状的二倍体植株自交后代中没有发现分离。因此,在本研究中所获得的所有花药诱导的二倍体植株都是纯合的,它们是由单倍体小孢子在离体发育时通过染色体加倍而产生的;在单倍体和二倍体植株中,正常叶对窄叶、有叶舌对无叶舌的比例都符合预期的1:1的配子分离比例。不过这四种基因型分组的次数完全不符合1:1:1:1的比率,表明nal和lg这两个基因是连锁的。从这两种倍数性的植株中获得的综合数据得出了9.28±1.47％的重组值。使用于花药培养同样的F_1自交产生F_2代,对F_2群体作进行分析,所得的结果与花药植株的结果完全一致。对本研究在遗传学和植物育种中的重要意义进行了讨论。
Genetic analysis of haploid and diploid plants induced by anther culture of hybrid rice (Oryza sativa L.2n = 24) with narrow leaves and no leaf tongue was performed. No segregation was found in the selfing offspring of diploid plants expressing dominant traits. Therefore, all of the anther-induced diploid plants obtained in this study were homozygous, which were generated by chromosome doubling of the haploid microspores upon in vitro development; at haploid and double In plants, the ratios of normal leaves to narrow leaves and those with ligule to no ligules all fit the expected 1: 1 gamete separation ratio. However, the number of these four genotyping groups did not match the ratio of 1: 1: 1: 1 at all, indicating that the two genes nal and lg are linked. The combined data obtained from these two multiplicative plants yielded a recombination value of 9.28 ± 1.47%. The F_2 generation was also produced by the same F_1 selfing in anther culture, and the F_2 population was analyzed. The results obtained were completely consistent with the results of anther plants. The significance of this study in genetics and plant breeding is discussed.