目的 :恶性肿瘤细胞逃避Anoikis凋亡的特性是其原位侵袭和远处转移的一个重要原因。本文旨在研究应用人干扰素 (IFN)α2a诱导骨肉瘤细胞出现Anoikis凋亡 ,并深入探讨其产生机理及信号传导途径。方法 :采用抑制接触培养法建立细胞Anoikis凋亡诱导模型。观测IFN α2a诱导骨肉瘤细胞MG 6 3产生的Anoikis凋亡。TUNEL法形态学检测MG 6 3细胞凋亡 ,流式细胞仪定量检测细胞凋亡程度和细胞表面整联蛋白受体表达。特异性底物切开法检测半胱氨酸天门冬氨酸特异性蛋白酶 (cysteineaspartate specificproteases ,caspase)信号途径。 结果 :人IFN α2a可明显诱导骨肉瘤细胞MG 6 3产生Anoikis现象 ,并诱导了MG 6 3细胞caspase 8和caspase 3的活性 ,但IFN对整联蛋白亚单位α4 ,α5及αv无明显影响 ,上述整联蛋白封闭抗体对IFNα 2a诱导的MG 6 3细胞Anoikis凋亡无明显影响。结论 :人干扰素α2a可以在体外通过调节细胞cas pase信号途径 ,诱导骨肉瘤细胞MG 6 3产生Anoikis现象 ,从而抑制骨肉瘤的转移 OBJECTIVE: The characteristic of malignant tumor cells escaping Anoikis apoptosis is an important reason for their in situ invasion and distant metastasis. This article aims to study the application of human interferon (IFN) α2a induced osteosarcoma Anoikis apoptosis, and to explore its mechanism of production and signal transduction pathway. Methods: Anoikis apoptosis induction model was established by inhibition of contact culture method. IFNα2a induced osteosarcoma cell line MG 6 3-induced Anoikis apoptosis. TUNEL method was used to detect the apoptosis of MG 6 3 cells. Flow cytometry was used to detect the degree of apoptosis and expression of cell surface integrin receptor. Specific substrate cleavage assay was used to detect the cysteine ​​aspartate specific protease (caspase) signaling pathway. RESULTS: Human IFNα2a significantly induced Anoikis in MG 6 3 cells and induced caspase 8 and caspase 3 activity in MG 6 3 cells. However, IFNα had no significant effect on integrin subunits α4, α5 and αv, The above integrin blocking antibodies had no significant effect on Anoikis apoptosis induced by IFNα 2a in MG 6 3 cells. Conclusion: Human interferon α2a can inhibit the osteogenesis of osteosarcoma by regulating the caspase signaling pathway in vitro and inducing the Anoikis phenomenon in osteosarcoma MG 6 3 cells