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目的探讨自拟解郁方对慢性不可预见性温和应激(chronic unpredictable mild stress,CUMS)致抑郁症大鼠下丘脑-垂体-肾上腺(hypothalamic-pituitary-adrenal,HPA)轴相关激素的影响。方法 2014年12月—2015年3月,选择40只大鼠随机分为对照组、模型组、氟西汀组、解郁方组各10只。模型组、氟西汀组、解郁方组采用CUMS构建抑郁模型,对照组及模型组给予生理盐水灌胃,氟西汀组给予氟西汀灌胃,解郁方组给予自拟解郁方灌胃,持续21 d。用药结束后,进行敞箱实验、糖水偏好实验;检测大鼠血清促肾上腺皮质激素释放激素(corticotropin releasing hormone,CRH)、促肾上腺皮质激素(adrenocorticotropic hormone,ACTH)、皮质酮(corticosterone,CORT)的含量。计量资料采用方差分析,两两比较采用LSD-t检验,P<0.05为差异有统计学意义。结果模型组水平穿越格数、竖立次数和糖水偏好水平分别为(44.18±2.60)个、(13.24±2.05)次、(0.549±0.019),明显降低对照组的(91.89±2.37)个、(23.46±2.13)次、(0.735±0.021),差异均有统计学意义(均P<0.05)。模型组CRH、ACTH、CORT水平分别为(72.62±7.98)pg/ml、(54.34±3.97)、(36.26±6.96)ng/ml,均高于对照组的(51.15±5.67)pg/ml、(40.27±3.16)、(23.75±6.34)ng/ml,差异均有统计学意义(均P<0.05)。与模型组相比,氟西汀组、解郁方组水平穿越格数、竖立次数和糖水偏好[(73.09±2.84)个、(19.26±2.14)次、(0.656±0.021)、(72.29±1.59)个、(19.19±2.17)次、(0.661±0.017)]明显增高,差异均有统计学意义(均P<0.05)。氟西汀组和解郁方组CRH、ACTH、CORT水平分别为(62.37±6.17)pg/ml、(45.61±3.25)、(25.61±5.35)ng/ml、(61.89±6.03)pg/ml、(45.31±3.34)、(25.37±5.26)ng/ml,均低于模型组,差异均有统计学意义(均P<0.05)。结论自拟解郁方对CUMS诱导的抑郁症大鼠HPA轴相关激素具有调节作用。
Objective To investigate the effects of Jieyu Decoction on the relevant hormones in hypothalamic-pituitary-adrenal (HPA) axis of chronic unpredictable mild stress (CUMS) -dependent depression rats. Methods From December 2014 to March 2015, 40 rats were randomly divided into control group, model group, fluoxetine group and Jieyufang group. The model group, the fluoxetine group and the Jieyufang group were treated with CUMS to establish the depression model, the control group and model group were given normal saline, the fluoxetine group was given fluoxetine, the Jieyufang group was given Jieyu prescription for 21 days . After the treatment, open-field test and sugar-water preference test were performed. Serum levels of corticotropin releasing hormone (CRH), adrenocorticotropic hormone (ACTH) and corticosterone (CORT) content. Measurement data were analyzed by ANOVA, pairwise comparisons using LSD-t test, P <0.05 for the difference was statistically significant. Results The number of horizontal traversal, erection and sugar preference in the model group were (44.18 ± 2.60), (13.24 ± 2.05) and (0.549 ± 0.019), respectively, which were significantly lower in the control group (91.89 ± 2.37) and (23.46 ± 2.13) times, (0.735 ± 0.021), the differences were statistically significant (all P <0.05). The levels of CRH, ACTH and CORT in the model group were (72.62 ± 7.98) pg / ml, 54.34 ± 3.97 and 36.26 ± 6.96 ng / ml, respectively, which were significantly higher than those in the control group (51.15 ± 5.67) pg / ml and 40.27 ± 3.16) and (23.75 ± 6.34) ng / ml, respectively (all P <0.05). Compared with the model group, the number of traversal grids, erection frequency and glycogen preference of fluoxetine group and Jieyufang group [(73.09 ± 2.84), (19.26 ± 2.14), (0.656 ± 0.021), (72.29 ± 1.59) , (19.19 ± 2.17) times, (0.661 ± 0.017)] respectively, all of which were significantly different (all P <0.05). The levels of CRH, ACTH and CORT in fluoxetine group and Jieyu group were (62.37 ± 6.17) pg / ml, (45.61 ± 3.25), (25.61 ± 5.35) ng / ml and 45.31 ± 3.34), (25.37 ± 5.26) ng / ml, all of which were lower than the model group (all P <0.05). Conclusion Self-made Jieyu Decoction can regulate the HPA axis related hormones in CUMS-induced depression rats.