【摘 要】
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Recently biospecific affinity chromatography has been widely used for the separaion and purification of various enzymes and nucleic acids. In this paper, a seri
【出 处】
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Chinese Chemical Letters
论文部分内容阅读
Recently biospecific affinity chromatography has been widely used for the separaion and purification of various enzymes and nucleic acids. In this paper, a series of synthetic reactions of solid-liquid phase were carried out on silica surface, using a macroporous(30μm),microspherical silica (8μm) as the matrix and γ-aminopropyltriethoxysilane as the activating agent,the nicotinamide adenine dinucleotide(NAD) was bonded through its amino groups to the carboxylic groups of linked phospholipid which was bonded covalently on aminated support. The bonded stationary phase has high thermal stability, and could be used to separae of nucleotides with good resolution
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