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目的:近来大量的研究证明肿瘤细胞以及肿瘤微环境中的巨噬细胞等分泌的TNF-α可促进包括乳腺癌在内的多种恶性肿瘤的转移。本研究拟在基因水平上较全面的了解TNF-α(20ng/ml)作用于乳腺癌细胞系(MCF-7)引起的转移相关基因的变化,以进一步探索TNF-α促进乳腺癌转移的机制。方法:Transwell法用于检测TNF-α作用于MCF-7细胞后引起的侵袭能力的变化;cDNA微阵列分析用来检测TNF-α作用于MCF-7细胞后转移相关基因的变化。结果:低剂量的TNF-α可提高MCF-7细胞的侵袭能力,cDNA微阵列分析显示39个转移相关基因的表达发生了明显变化,TNF-α对其中6个基因之间的调节关系目前尚无相关报道。功能分析显示大多数的基因促进MCF-7细胞的转移,另外少数基因则抑制其转移。TNF-α作用后的这些基因表达变化有一定的时间依赖特点。结论:低剂量的TNF-α可通过影响一系列转移相关基因,从而增强MCF-7细胞的侵袭能力。研究中发现的一些表达变化明显的基因值得进一步的研究。
PURPOSE: Recently, a large number of studies have demonstrated that the secretion of TNF-α by tumor cells and macrophages in the tumor microenvironment can promote the metastasis of many malignant tumors including breast cancer. This study intends to investigate gene expression of metastasis-related genes induced by TNF-α (20ng / ml) in breast cancer cell line (MCF-7) in order to further explore the mechanism of TNF-α promoting breast cancer metastasis . Methods: Transwell method was used to detect the changes of invasiveness induced by TNF-α in MCF-7 cells. CDNA microarray analysis was used to detect the changes of metastasis-related genes after TNF-α treatment. Results: The low dose of TNF-α could enhance the invasion ability of MCF-7 cells. The cDNA microarray analysis showed that there were significant changes in the expression of 39 metastasis-related genes. The regulatory effect of TNF-α on 6 genes No relevant reports. Functional analysis shows that most of the genes promote the metastasis of MCF-7 cells, while a few genes inhibit their metastasis. The expression of these genes after TNF-α has some time-dependent characteristics. Conclusion: Low-dose TNF-α can enhance the invasiveness of MCF-7 cells by affecting a series of metastasis-related genes. Some of the genes found in the study that show significant changes deserve further study.