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目的建立高效液相色谱仪同时检测蔬菜中噻苯隆、氯吡脲、脱落酸、6-糖基氨基嘌呤、N6-异戊烯腺嘌呤、6-苄氨基嘌呤、吲哚-3-丁酸、吲哚-3-乙酸、α-萘乙酸、α-萘乙酸甲酯10种植物生长调节剂残留量的方法。方法乙腈提取样品中的待测物,蒸发除去多余乙腈。残渣用二氯甲烷溶解,溶液过氨基柱净化,用甲醇洗脱分析物,浓缩后,以Agilent C18为分析柱,0.1%甲酸-甲醇进行梯度洗脱,紫外/荧光检测器同时检测。以保留时间定性,标准曲线外标法定量。结果 10种植物植物生长调节剂的检出限为1.0μg/kg~8.0μg/kg,线性范围0.020μg/ml~5.00μg/ml,相关系数r均>0.999,回收率为70%~104%,精密度RSD均<4.0%。结论本方法操作简便、灵敏度高、重现性好,应用于日常检测可大大降低检测成本,缩短检测周期,适合基层推广。
OBJECTIVE To establish a high performance liquid chromatograph (HPLC) for the simultaneous determination of thidiazuron, clopidamide, abscisic acid, 6-glycosylaminopurine, N6-isopentenyladenine, 6-benzylaminopurine, indole- , Indole-3-acetic acid, α-naphthalene acetic acid, methyl α-naphthylacetate 10 kinds of plant growth regulators. Methods Acetonitrile was used to extract analytes from the sample and evaporated to remove excess acetonitrile. The residue was dissolved in methylene chloride and the solution was purified with an amino column. The analyte was eluted with methanol. After concentration, the residue was eluted with an Agilent C18 column and eluted with 0.1% formic acid-methanol. The UV / fluorescence detector was simultaneously detected. To retain the time qualitative, standard curve external standard method. Results The detection limits of 10 plant growth regulators ranged from 1.0μg / kg to 8.0μg / kg and the linear range was from 0.020μg / ml to 5.00μg / ml with correlation coefficients r> 0.999. The recoveries ranged from 70% to 104% , RSD precision <4.0%. Conclusion The method is simple, sensitive, reproducible and can be applied to routine testing to greatly reduce testing costs, shorten the testing cycle and be suitable for grassroots promotion.