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目的:探讨髓系抑制性细胞(MDSCs)及诱导型一氧化氮合酶(iNOS)在哮喘小鼠肺组织中的表达及意义。方法:将BALB/C小鼠随机分成哮喘组及对照组,每组10只。用卵清蛋白(OVA)致敏、激发建立小鼠哮喘模型,用HE染色观察两组小鼠肺组织病理改变;用免疫组织化学(免疫组化)方法检测小鼠肺组织中MDSCs积分光密度值平均值;用RT-PCR检测小鼠肺组织中iNOS mRNA的表达。结果:哮喘组可见气道壁增厚,炎性细胞浸润,对照组则无明显变化。哮喘组小鼠肺组织MDSCs、iNOS mRNA的表达水平显著高于对照组,差异有统计学意义(P<0.05)。结论:哮喘小鼠肺组织中MDSCs的聚集增加,iNOS的表达增多,MDSCs通过表达iNOS来实现其在哮喘小鼠气道炎症、气道高反应及免疫抑制等方面的重要作用。
Objective: To investigate the expression and significance of myeloid suppressor cells (MDSCs) and inducible nitric oxide synthase (iNOS) in lung tissue of asthmatic mice. Methods: BALB / C mice were randomly divided into asthma group and control group, with 10 mice in each group. Mice were sensitized with ovalbumin (OVA) to establish a mouse model of asthma. The pathological changes of the lungs of the two groups were observed by HE staining. The optical density of MDSCs in the lungs of mice was detected by immunohistochemistry (IHC) The mean value of iNOS mRNA in lung tissue was detected by RT-PCR. Results: Airway wall thickening and inflammatory cell infiltration were found in the asthma group, but there was no obvious change in the control group. The expression of MDSCs and iNOS mRNA in lung tissue of asthmatic mice was significantly higher than that of the control group (P <0.05). CONCLUSIONS: The accumulation of MDSCs and the increase of iNOS in the lungs of asthmatic mice play an important role in the airway inflammation, airway hyperresponsiveness and immunosuppression in asthmatic mice through the expression of iNOS.