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由乙肝病毒S基因转化的哺乳动物细胞培养收液，经过Butyl-S-SepharoseFF层析，DEAESepharoseFF层析和Sepharose4FF层析，可获HBsAg纯品。产品检定结果表明，PAGE和SDS-PAGE电泳纯度均合格，小牛血清残余量、细胞DNA残余量和动物免疫效力也均符合规定标准．HBsAg终收率达40％左右．在此工艺中，疏水作用柱层析的纯化效率比较高，可去除绝大部分杂蛋白和细胞DNA。
Mammalian cells transformed with Hepatitis B virus S gene were harvested and purified by Butyl-S-Sepharose FF chromatography, DEAE Sepharose FF chromatography and Sepharose 4FF chromatography to obtain pure HBsAg. The product test results showed that PAGE and SDS-PAGE electrophoresis purity were qualified, the residual amount of bovine serum, residual DNA and animal immune efficiency also meet the required standards. HBsAg final yield of about 40%. In this process, the hydrophobic purification column chromatography purification efficiency is relatively high, can remove most of the hybrid protein and cell DNA.